STEREOLOGY WORKSHOP August 2012

Provisional program (may be subject to change):

1. Week: Stereology

Monday
Presentation of projects by participants
Lecture: Definition of probes, sampling and basic statistics

Lecture: Volume estimation, Cavalieri principle
Exercise: Volume estimation
Practical: Agar embedding and slicing of organs

Tuesday
Lecture: The fractionator principle (systematic sampling)
Exercise: The fractionator
Lecture: The disector, counting and sampling in 3D
Exercise: Number estimation

Lecture: More disector sampling, including connectivity
Exercise: Connectivity
Exercise: Alveolar number

Wednesday
Lecture: Length and Surface, IUR
Exercise: Length estimation
Lecture: Ratios, multilevel sampling design, reference trap

Lecture: Vertical sections
Exercise: Surface from vertical sections
Practical: Fractionator sampling, vertical sections; embedding, sectioning and staining

Thursday
Lecture: Sampling and sizing of particles
Exercise: The Nucleator
Exercise: Point sampled intercepts

Lecture: Tissue deformation, slicing, tools and gadgets
Demonstration: Stereology and imaging machinery: newCAST
Demonstration: Stereology web-tool: the STEPanizer

Friday
Lecture: All the estimators of particle size
Lunch break
Final presentation of projects by participants

Weekend:

Hands-on computer based stereology and imaging machinery:

    newCAST (Visiopharm, DK)

Two days with practical exercise